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  • Pseudoalignment for single cell RNA-seq with alevin-fry

    calendar Jul 28, 2025 · 4 min read · code single cell  ·
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    Pseudoalignment for single cell RNA-seq with alevin-fry

    Alevin-fry: pseudoalignment for computationally eficient single cell RNA-seq quantification


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  • Pairwise Controlled Manifold Approximation (PaCMAP) for Single Cell

    calendar Jun 5, 2025 · 21 min read · dimensional reduction code single cell  ·
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    Pairwise Controlled Manifold Approximation (PaCMAP) for Single Cell

    Dimensional Reduction for Single Cell new player: PaCMAP


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  • Single Cell Dimensional Reduction: Phate

    calendar May 25, 2025 · 22 min read · code single cell dimensional reduction phate  ·
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    Single Cell Dimensional Reduction: Phate

    There is more than just tSNE and UMAP in Single Cell Dimensional Reduction. In this post I will check Phate.


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  • Single Cell Dimensional Reduction: t-SNE vs UMAP

    calendar May 16, 2025 · 11 min read · code single cell dimensional reduction t-SNE UMAP  ·
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    Single Cell Dimensional Reduction: t-SNE vs UMAP

    t-SNE & UMAP dimensional reduction for single cell data. How to choose between them?


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  • Pseudotime Trajectory vs RNA Velocity

    calendar May 13, 2025 · 14 min read · code Pseudotime single cell RNA velocity Trajectory analysis Bioconductor  ·
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    Pseudotime Trajectory vs RNA Velocity

    Pseudotime trajectories and RNA velocity are computational approaches to analyze single cell dynamical process but, do they tell the same history?


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  • Understanding Cellular Dynamics with RNA Velocity

    calendar Apr 28, 2025 · 7 min read · RNA velocity scVelo scanpy Pseudotime single cell RNAseq  ·
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    Understanding Cellular Dynamics with RNA Velocity

    How to use scVelo to explore gene expression dynamics and differentiation


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  • Pseudotime Trajectories with Slingshot

    calendar Apr 24, 2025 · 12 min read · code Trajectory analysis Pseudotime single cell RNAseq Bioconductor  ·
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    Pseudotime Trajectories with Slingshot

    Using Slingshot to calculate single cell trajectories


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  • Single Cell PCA Deep Dive

    calendar Apr 16, 2025 · 7 min read · single cell code Bioconductor  ·
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    Single Cell PCA Deep Dive

    PCA is the first step of single cell data analysis, but how many PCs should you use?


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  • ENACT Visium HD cell segmentation and annotation

    calendar Apr 9, 2025 · 13 min read · Python Spatial Cell Segmentation Cell Annotation  ·
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    ENACT Visium HD cell segmentation and annotation

    See ENACT, a complete end-to-end pipeline that includes Visium HD Cell Segmentation and Cell type annotation, in action.


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  • Visium HD cell segmentation with Bin2Cell

    calendar Mar 26, 2025 · 16 min read · Python Spatial Cell Segmentation  ·
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    Visium HD cell segmentation with Bin2Cell

    Moving beyond nuclei segmentation, this post explores Bin2Cell for full cell segmentation in Visium HD spatial data.


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Alfonso Saera Vila

Welcome to 'Love, DNA & Code' my blog about new bioinformatics tools, single-cell & spatial applications, AI, and more.
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Featured Posts

  • Visium HD cell segmentation with Bin2Cell
  • Single cell in Python: Scanpy and AnnData

Recent Posts

  • Pseudoalignment for single cell RNA-seq with alevin-fry
  • Pairwise Controlled Manifold Approximation (PaCMAP) for Single Cell
  • Single Cell Dimensional Reduction: Phate
  • Single Cell Dimensional Reduction: t-SNE vs UMAP
  • Pseudotime Trajectory vs RNA Velocity
  • Understanding Cellular Dynamics with RNA Velocity
  • Pseudotime Trajectories with Slingshot
  • Single Cell PCA Deep Dive

Categories

BIOINFORMATICS 14 R 9 PYTHON 6 TECHNOLOGY 5

Tags

CODE 11 SINGLE CELL 11 BIOCONDUCTOR 4 RNASEQ 4 CELL SEGMENTATION 3 DIMENSIONAL REDUCTION 3 PSEUDOTIME 3 PYTHON 3 SPATIAL 3 AI 2 RNA VELOCITY 2 TRAJECTORY ANALYSIS 2 CELL ANNOTATION 1 CHATGPT 1
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AI2 BIOCONDUCTOR4 CELL ANNOTATION1 CELL SEGMENTATION3 CHATGPT1 CODE11 DEEP LEARNING1 DIMENSIONAL REDUCTION3 INDEX1 LLM1 PHATE1 PLOTS1 PSEUDOTIME3 PYTHON3 RNA VELOCITY2 RNASEQ4 SCANPY1 SCVELO1 SEURAT1 SINGLE CELL11 SPATIAL3 T-SNE1 TENSORFLOW1 TRAJECTORY ANALYSIS2 UMAP1
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Copyright © 2008–2025, Alfonso Saera plus the clarity & Hugo Authors

Copyright  COPYRIGHT © 2008–2025, ALFONSO SAERA PLUS THE CLARITY & HUGO AUTHORS. All Rights Reserved

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