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  • Find Marker Genes Using Effect Size

    calendar Aug 6, 2026 · 38 min read · single cell Marker Genes Differential Expression Analysis P-value Cohen's d  ·
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    Find Marker Genes Using Effect Size

    Finding marker genes in your single cell dataset may not be as simple as as you thought


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  • From Prompt to Pipeline: Building a scRNA-seq Workflow with Claude Code and Nextflow

    calendar Feb 10, 2026 · 9 min read · AI Deep Learning LLM Code Claude single cell RNAseq  ·
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    From Prompt to Pipeline: Building a scRNA-seq Workflow with Claude Code and Nextflow

    What happens when you ask an AI coding agent to build a complete scRNA-seq Nextflow pipeline from scratch? I put Claude Code to the test and share the results, the struggles, and the takeaways.


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  • Autoencoders for Single Cell Data Analysis

    calendar Jan 5, 2026 · 48 min read · AI Deep Learning dimensional reduction single cell RNAseq  ·
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    Autoencoders for Single Cell Data Analysis

    Explore the use of autoencoders to compress gene expression data and improve data analysis and visualization.


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  • BiocPy: Bioconductor in Python

    calendar Nov 25, 2025 · 8 min read · Bioconductor single cell  ·
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    BiocPy: Bioconductor in Python

    A quick tour of biocpy, the first Python framework to deliver Bioconductor-style data structures and workflows for genomic data analysis.


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  • Building Your First Nextflow Pipeline: PART 4 - Polish your pipeline

    calendar Aug 31, 2025 · 8 min read · code single cell pseudoalignment RNAseq  ·
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    Building Your First Nextflow Pipeline: PART 4 - Polish your pipeline

    Learn how to build bioinformatics pipelines with Nextflow, a powerful workflow management system for reproducible and scalable data analysis. PART 4 - Polish your pipeline


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  • Building Your First Nextflow Pipeline: PART 3 - Modularization & Configurable settings

    calendar Aug 30, 2025 · 5 min read · code single cell pseudoalignment RNAseq  ·
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    Building Your First Nextflow Pipeline: PART 3 - Modularization & Configurable settings

    Learn how to build bioinformatics pipelines with Nextflow, a powerful workflow management system for reproducible and scalable data analysis. PART 3 - Modularization & Configurable settings


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  • Building Your First Nextflow Pipeline: PART 2 - Multiple Samples

    calendar Aug 27, 2025 · 9 min read · code single cell pseudoalignment RNAseq  ·
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    Building Your First Nextflow Pipeline: PART 2 - Multiple Samples

    Learn how to build bioinformatics pipelines with Nextflow, a powerful workflow management system for reproducible and scalable data analysis. PART 2 - Multiple Samples


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  • Alignment or Pseudoalignment: Alevin-fry vs Cell Ranger comparison

    calendar Aug 6, 2025 · 9 min read · Bioconductor code single cell RNAseq  ·
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    Alignment or Pseudoalignment: Alevin-fry vs Cell Ranger comparison

    Compare Alevin-fry’s pseudoalignment and Cell Ranger’s full alignment to find the right single-cell RNA-seq tool for your pipeline.


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  • Building Your First Nextflow Pipeline: PART 1 - One sample at a time

    calendar Jul 30, 2025 · 8 min read · code single cell pseudoalignment RNAseq  ·
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    Building Your First Nextflow Pipeline: PART 1 - One sample at a time

    Learn how to build bioinformatics pipelines with Nextflow, a powerful workflow management system for reproducible and scalable data analysis.


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  • Pseudoalignment for single cell RNA-seq with alevin-fry

    calendar Jul 28, 2025 · 4 min read · code single cell  ·
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    Pseudoalignment for single cell RNA-seq with alevin-fry

    Alevin-fry: pseudoalignment for computationally eficient single cell RNA-seq quantification


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Alfonso Saera Vila

Welcome to 'Love, DNA & Code' my blog about new bioinformatics tools, single-cell & spatial applications, AI, and more.
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Featured Posts

  • Find Marker Genes Using Effect Size
  • Skills: Teach Claude Code Bioinformatics
  • From Prompt to Pipeline: Building a scRNA-seq Workflow with Claude Code and Nextflow
  • Claude Code for Bioinformaticians: Your AI Coding Assistant in the Terminal
  • BiocPy: Bioconductor in Python
  • Building Your First Nextflow Pipeline: PART 4 - Polish your pipeline
  • Single Cell Dimensional Reduction: t-SNE vs UMAP
  • Pseudotime Trajectory vs RNA Velocity

Recent Posts

  • OpenClaw Setup
  • The Basics of Matrix Multiplication
  • Autoencoders for Single Cell Data Analysis
  • Bringing the Tidyverse to Python: plotnine, siuba, and pyjanitor
  • Use uv for python and forget about anything else!
  • Building Your First Nextflow Pipeline: PART 3 - Modularization & Configurable settings
  • Building Your First Nextflow Pipeline: PART 2 - Multiple Samples
  • Alignment or Pseudoalignment: Alevin-fry vs Cell Ranger comparison

Categories

BIOINFORMATICS 23 R 13 PYTHON 12 CODE 6 TECHNOLOGY 6 NEXTFLOW 5 BIONFORMATICS 1 OPENCLAW 1

Tags

CODE 22 SINGLE CELL 20 RNASEQ 11 AI 8 BIOCONDUCTOR 6 DEEP LEARNING 5 DIMENSIONAL REDUCTION 4 PSEUDOALIGNMENT 4 CELL SEGMENTATION 3 CLAUDE 3 LLM 3 PSEUDOTIME 3 PYTHON 3 SPATIAL 3
All Tags
AGENT1 AI8 BIOCONDUCTOR6 CELL ANNOTATION1 CELL SEGMENTATION3 CHATGPT1 CLAUDE3 CODE22 COHEN'S D1 DEEP LEARNING5 DIFFERENTIAL EXPRESSION ANALYSIS1 DIMENSIONAL REDUCTION4 INDEX1 LLM3 MARKER GENES1 OPENCLAW1 P-VALUE1 PHATE1 PLOTS2 PRODUCTIVITY1 PSEUDOALIGNMENT4 PSEUDOTIME3 PYTHON3 RNA VELOCITY2 RNASEQ11 SCANPY1 SCVELO1 SEURAT1 SINGLE CELL20 SPATIAL3 T-SNE1 TENSORFLOW1 TRAJECTORY ANALYSIS2 UMAP1 UV1
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Copyright © 2008–2025, Alfonso Saera plus the clarity & Hugo Authors

Copyright  COPYRIGHT © 2008–2025, ALFONSO SAERA PLUS THE CLARITY & HUGO AUTHORS. All Rights Reserved

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